The USS Cole bombing was an act of terrorism against USS Cole, a guided missile destroyer of the United States Navy, on 12 October 2000, while she was being refueled in Yemen's Aden harbor. On the morning of Thursday, 12 October 2000, Cole, under the command of Commander Kirk Lippold, docked in Aden harbor for a routine fuel stop. Cole completed mooring at 9:30 and began refueling at 10:30. Around 11:18 local time, a small fiberglass boat carrying C4 explosives and two suicide bombers approached the port side of the destroyer and exploded, creating a 40-by-60-foot gash in the ship's port side, according to the memorial plate to those who lost their lives.

Former CIA intelligence officer Robert Finke said the blast appeared to be caused by C4 explosives molded into a shaped charge against the hull of the boat. Around 400 to 700 pounds of explosive were used. Much of the blast entered a mechanical space below the ship's galley, violently pushing up the deck, thereby killing crew members who were lining up for lunch. The crew fought flooding in the engineering spaces and had the damage under control after three days. Divers inspected the hull and determined that the keel had not been damaged.

Seventeen U.S. Navy sailors were killed and 39 injured in the deadliest attack against a United States naval vessel since the USS Stark incident in 1987.

The Militant Islam organization al-Qaeda claimed responsibility for the attack against the United States. A U.S. judge has held Sudan liable for the attack, while another has released over $13 million in Sudanese frozen assets to the relatives of those killed. The United States Navy has reconsidered their rules of engagement in response to this attack.

The attack is widely seen as a precursor to the September 11 attacks.